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rnase h  (New England Biolabs)


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    Structured Review

    New England Biolabs rnase h
    Rnase H, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnase+i/DNA+Polymerase+I/pmc13090509-34-33-35
    Average 99 stars, based on 10389 article reviews
    rnase h - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: AAV-delivered engineered suppressor tRNA rescues visual function in mice with an inherited retinal disease.
    Article Snippet: .. To prepare RFs, 10μL of RNase I (NEB, Ipswich, MA, USA) and 6μL of DNase I (NEB, Ipswich,MA, USA)were added to 400μL of lysate, which was then incubated for 45min at roomtemperaturewith gentlemixing on aNutatormixer.Nuclease digestionwas stopped by adding 10μL of SUPERase·In RNase inhibitor (Ambion, Austin, TX, USA). .. Size exclusion columns (Illustra MicroSpin S-400 HR Columns; GE Healthcare; Cat. No. 27- 5140-01) were equilibrated with 3mL of polysome buffer by gravity flow and centrifuged at 600 g for 4min at room temperature.

    Article Title: Identification of cellular double-stranded RNAs in mammalian embryonic stem cells
    Article Snippet: .. Beads were washed three times with cold IP buffer and incubated with either 100U RNase I (M0243S, NEB) or 2U RNAse III (M0245S, NEB) 100 μl of the manufacturer’s supplied buffers for 20’ at 37°C. ..

    Article Title: Multi-Tissue Omics Analysis Uncovers Translational Regulation Underlying Complex Traits in Pigs.
    Article Snippet: The lysate was incubated on ice for 20 min and then clarified by centrifugation Advanced Science, 2026 at 20 000 × g for 3 min at 4◦C. .. For each sample, 10 μL RNase I (NEB, M0307) and 6 μL DNase I (NEB, M0303) were added and incubated for 45 min at room temperature. .. Nuclease digestion was halted by adding 10 μL of SUPERase ⋅In RNase inhibitor (Invitrogen, AM2694).

    Article Title: Dysregulated proline metabolism exacerbates HCC metastasis via EPRS1-mediated mRNA translation
    Article Snippet: .. For ribosome footprints (RFs) preparation, RNase I (Cat. #M0307, NEB) and DNase I (Cat. #M0303, NEB) were added to 400 μL of lysate and then gently mixed on a nutator mixer at room temperature and incubated for 45 min. Nuclease digestion was stopped by adding 10 μL SUPERase·InTM RNase inhibitor (Cat. #AM2696, Invitrogen). .. The size-exclusion column (Cat. #27-5140-01, GE HealthCare) was equilibrated by gravity flow with 3 mL of polysome buffer and then centrifuged at 600 ×g for 4 min at room temperature.

    Article Title: Multi‐Tissue Omics Analysis Uncovers Translational Regulation Underlying Complex Traits in Pigs
    Article Snippet: .. For each sample, 10 μL RNase I (NEB, M0307) and 6 μL DNase I (NEB, M0303) were added and incubated for 45 min at room temperature. .. Nuclease digestion was halted by adding 10 μL of SUPERase·In RNase inhibitor (Invitrogen, AM2694).

    Article Title: AAV-delivered engineered suppressor tRNA rescues visual function in mice with an inherited retinal disease
    Article Snippet: .. To prepare RFs, 10 μL of RNase I (NEB, Ipswich, MA, USA) and 6 μL of DNase I (NEB, Ipswich, MA, USA) were added to 400 μL of lysate, which was then incubated for 45 min at room temperature with gentle mixing on a Nutator mixer. .. Nuclease digestion was stopped by adding 10 μL of SUPERase·In RNase inhibitor (Ambion, Austin, TX, USA).

    Article Title: mRNA Imprinting: transcription apparatus can remotely control cytoplasmic post-transcriptional mechanisms by dozens of proteins
    Article Snippet: Beads were washed 4X with 1ml wash buffer (50 mM Tris HCl, pH 7.4, with 150 mM NaCl, 10mM EDTA) supplemented with Protease inhibitors Complete (Roche). .. Beads were incubated with wash buffer with 500 units RNase I (New England Biolabs) for 3 hours at room temperature with gentle agitation. ..

    Gentle:

    Article Title: AAV-delivered engineered suppressor tRNA rescues visual function in mice with an inherited retinal disease
    Article Snippet: .. To prepare RFs, 10 μL of RNase I (NEB, Ipswich, MA, USA) and 6 μL of DNase I (NEB, Ipswich, MA, USA) were added to 400 μL of lysate, which was then incubated for 45 min at room temperature with gentle mixing on a Nutator mixer. .. Nuclease digestion was stopped by adding 10 μL of SUPERase·In RNase inhibitor (Ambion, Austin, TX, USA).

    Article Title: mRNA Imprinting: transcription apparatus can remotely control cytoplasmic post-transcriptional mechanisms by dozens of proteins
    Article Snippet: Beads were washed 4X with 1ml wash buffer (50 mM Tris HCl, pH 7.4, with 150 mM NaCl, 10mM EDTA) supplemented with Protease inhibitors Complete (Roche). .. Beads were incubated with wash buffer with 500 units RNase I (New England Biolabs) for 3 hours at room temperature with gentle agitation. ..



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